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Identifying Functional Epitopes In Site 1 Of Human Prolactin Hormone

Vittal Rao, Geeta
http://rave.ohiolink.edu/etdc/view?acc_num=osu1226014118

Abstract Details

2008, Master of Science, Ohio State University, Biophysics.
An increasing body of evidence suggests that human prolactin (hPRL) is involved in the growth and survival of breast and prostate tumors. This has increased interest in rationally engineered antagonists, for which functional epitopes in hormone and receptor need to be mapped. The goals of this project were to identify the functional epitopes in site 1 of hPRL and to quantify the kinetic and energetic contributions of these residues when bound to the hPRLr. This was accomplished by generating 102 hPRL alanine mutants and by studying the changes in binding affinities and free energies of these mutants when bound to hPRLr relative to wild-type hPRL. This work has identified a number of functionally important residues that reside within and outside the hPRL site 1 binding interface. Additionally, this study provides strong support that hPRL is a highly cooperative molecule and also provides leads for design of hPRL antagonists with enhanced site 1 binding affinity.
Charles Brooks (Advisor)
Charles Bell (Committee Member)
Donald Dean (Committee Member)
199 p.
Biochemistry; Biophysics
human prolactin; site 1; functional epitopes; antagonist

Recommended Citations

Citations

  • Vittal Rao, G. (2008). Identifying Functional Epitopes In Site 1 Of Human Prolactin Hormone [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1226014118

    APA Style (7th edition)

  • Vittal Rao, Geeta. Identifying Functional Epitopes In Site 1 Of Human Prolactin Hormone. 2008. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1226014118.

    MLA Style (8th edition)

  • Vittal Rao, Geeta. "Identifying Functional Epitopes In Site 1 Of Human Prolactin Hormone." Master's thesis, Ohio State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=osu1226014118

    Chicago Manual of Style (17th edition)

osu1226014118
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© 2008, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.